Acetylgestagenes

Medroxyprogesterone acetate (MPA), 17a-acetoxyprogesterone, megestrol acetate, chloromadinone acetate and melengestrol acetate belong to the group of acetylgestagenes.

Acetylgestagenes are known to increase the efficiency of animal production and have been used in illegal cocktails for calf fattening. These synthetic hormones are not permitted as resource for animal fattening. Some studies have shown that the peak concentrations of acetylgestagene residues can be observed in fat tissue. MPA, for instance, can be detected in plasma only a few days past application but it cannot be detected with accuracy in urine. It is a fact that after the application of MPA to calves high concentrations of non-metabolized MPA can be detected in fat, even after 19 days.

Using the RIDASCREEN® Acetylgestagene assay, it is possible to detect these synthetic hormones in bovine fat both rapidly and with accuracy.

<b>Format:</b>
96 well microtitreplate
 
<b>Detection limit:</b>
MPA 30 ppt
17a-Acetoxyprogesterone 24 ppt
Megestrol acetate 60 ppt
Chloromadinone acetate 60 ppt
Melengestrol acetate 5 ppt
 
<b>Time requirement:</b>
incubation time 2 h 30 min
 
<b>Sample preparation:</b>
over night extraction, freezing, centrifugation, repeated extraction, freezing, centrifugation, drying, washing and chromatographic prepurification by means of RIDA® C18 columns (R 2002)
 
<b>Recovery rate:</b>
50 %
 
<b>Cross reaction:</b>
MPA 100 %
17a-Acetoxyprogesterone 126 %
Megestrol acetate 50 %
Chloromadinone acetate 50 %
Melengestrol acetate 0.6 %
all other cross reactions are 0.02 % or < 0.02 %
 
<b>Samples per kit:</b>
up to 42 in duplicate
 
<b>Standards:</b>
0 / 150 / 450 / 1,350 / 4,050 / 12,150 ppt
 
<b>Measurement: </b>
microtiter plate reader (450 nm)