Deoxynivalenol (DON)

Deoxynivalenol belongs to the trichothecene group of mycotoxins and is formed by fungi of the genus Fusarium. Deoxynivalenol often occurs in plant products particularly in cereals. Of the trichothecene mycotoxins deoxynivalenol, 3-acetyldeoxynivalenol and 15-acetyl-deoxynivalenol are the toxins most frequently occuring in Europe and Northern America. The toxin concentrations found in wheat, corn or rice are often in the ppm range. Due to their high cytotoxic and immunosuppressive properties, these toxins pose a risk to human and animal health.

Using the RIDASCREEN® DON assay, it is possible to detect this mycotoxin in cereals, feed and beer both rapidly and with accuracy.

Since the antibody used in the assay is specific for acetylated deoxynivalenol, the samples have to be acetylated after extraction. During acetylation, deoxynivalenol reacts to 3-acetyl-deoxynivalenol, 15- acetyldeoxynivalenol and 3, 15-diacetyl-deoxynivalenol. The assay can detect all of these toxins and will be positive, if at least one of these toxins is present. Thus, if a sample contains more than one of these toxins, the assay result will represent the sum of those toxins.
 
   
<b>Format:</b>
96 well microtitreplate
 
<b>Detection limit: </b>
1.25 µg/kg (ppb)
 
<b>Time requirement: </b>
incubation time 2 h 30 min
(regardless of the number of samples)
 
<b>Sample preparation: </b>
Cereals/feed: sample trituration, extraction, filtration, evaporation, acetylation and dilution
Beer: extraction, centrifugation, evaporation, acetylation and chromatographic purification by
means of RIDA® C18 columns (R2002)
 
<b>Recovery rate: </b>
Cereals/feed approx. 80 - 90 %
 
<b>Cross reaction: </b>
DON (acetylated) 100 %
3-Acetyldeoxynivalenol 100 %
15-Acetyldeoxynivalenol 100 %
Acetyl-T-2 toxin 0.25 %
T-2 tetraoltetraacetate 0.13 %
Nivalenol (acetylated) 0.1 %
all other cross reactions are < 0.1 %
 
<b>Samples per kit: </b>
up to 42 in duplicate
 
<b>Standards: </b>
0 / 25 / 50 / 100 / 200 / 400 ppt
 
<b>Measurement:  </b>
microtiter plate reader (450 nm)
 
For RIDASCREEN® Aflatoxin B1, Aflatoxin Total, Ochratoxin A, Zearalenon and DON a common sample preparation method for cereals and grain is available on request.