19-nortestosterone

The anabolic effects of 19-nortestosterone are comparable to those of testosterone, but with less androgenic effects.

In Germany, 19-nortestosterone-decanate and -laureate are permitted for veterinary use.

However, the illegal use of 19-nortestosterone as doping agents in high performance sports or horse sports are far more common. Illegal cocktails as a fattening resource in calf fattening usually contain 19-nortestosterone-ester.

Using the RIDASCREEN® 19-Nortestosteron assay, it is possible to detect this anabolic steroid in urine and meat both rapidly and with accuracy. 
 
 
Format: 96 well microtiterplate 
 
Detection limit:
800 ng/kg (ppt) for urine samples
400 ng/kg (ppt) for meat samples
 
Time requirement: incubation time 2 h 30 min
 
Sample preparation:
hydrolysis (urine) or tert-butylmethylether extraction (meat) chromatographic prepurification by means of RIDA© C18 columns (R 2002)
 
Recovery rate: urine 85 %
 
Cross reaction:
19-Nortestosterone 17ß 100 %
19-Norandrostendione 100 %
19-Nortestosterone 17a 80 %
19-Norethisterone 74 %
15a, 16a-Methylene-19NT-17ß-acetate 71 %
18-Methyl-19 NT-17ß 59 %
17a-Ethyl-19 NT-17ß 40 %
Trenbolone 10 %
all other cross reactions are < 0.1 %
 
Samples per kit: up to 42 in duplicate
 
Standards: 0 / 200 / 600 / 1,800 / 5,400 / 16,200 ppt
 
Measurement: microtiter plate reader (450 nm)