Chloramphenicol

Chloramphenicol is a broad spectrum antibiotic which is frequently employed in animal production for its excellent antibacterial and pharmacokinetic properties. However, in humans it leads to hematotoxic side effects, in particular chloramphenicol induced aplastic anaemia for which a dosage effect relationship has not yet been established. This has led to a prohibition of chloramphenicol for the treatment of animals used for food production.

Using the RIDASCREEN® Chloramphenicol assay, it is possible to detect this antibiotic in milk, meat and eggs both rapidly and with accuracy.

<b>Format:</b>
96 well microtitreplate
 
<b>Detection limit:</b>
milk 150 ng/l (ppt)
meat and eggs 100 ng/kg (ppt)
 
<b>Time requirement:</b>
Incubation time 2 h 30 min (regardless of the number of samples)
 
<b>Sample preparation:</b>
milk: centrifugation for defatting, precipitation with Carrez I / II, centrifugation and dilution meat/eggs: ethyl acetate extraction, evaporation and defatting
 
<b>Recovery rate:</b>
milk 84 %
meat and eggs 76 %
 
<b>Cross reaction:</b>
Chloramphenicol 100 %
Chloramphenicol base 0.5 %
Thiamphenicol < 0.05 %
no cross reactions with tetracycline, gentamicin and ampicillin
 
<b>Samples per kit:</b>
up to 42
 
<b>Standards:</b>
0 / 500 / 1,500 / 4,500 / 13,500 / 40,500 ppt
 
<b>Measurement: </b>
microtiter plate reader (450 nm)