17ß-estradiol

17ß-estradiol is a natural sexual hormone.

The levels of 17ß-estradiol in bovine plasma from peripheral blood are very low. In calves not more than 1 - 2 pg/ml are present and in cycling females the concentration of 17ß-estradiol is 1 - 5 pg/ml.

Only in plasma of pregnant animals or in plasma of animals illegally treated with anabolic agents, levels in the range of 100 to 1,000 pg/ml can be found.

Using the RIDASCREEN® 17ß-estradiol assay it is possible to detect this natural hormone in plasma both rapidly and with accuracy. 
 
Format: 96 well microtiterplate
 
Detection limit: 20 ng/kg (ppt) for plasma samples
 
Time requirement: incubation time 2 h 30 min
 
Sample preparation: extraction, freezing and evaporation
 
Recovery rate: plasma approx. 85 %
 
Cross reaction:
17ß-Estradiol 100 %
Estradiol-3-benzoate 50 %
Trenbolone 1 %
all other cross reactions are < 1 %
 
Samples per kit: up to 42 in duplicate
 
Standards: 0 / 50 / 200 / 800 / 3,200 / 12,800 ppt
 
Measurement: microtiter plate reader (450 nm)