Chloramphenicol glucuronide

Chloramphenicol is a broad spectrum antibiotic which is frequently employed in animal production for its excellent antibacterial and pharmacokinetic properties. However, in humans it leads to hematotoxic side effects, in particular chloramphenicol induced aplastic anaemia for which a dosage effect relationship has not yet been established. This has led to a prohibition of chloramphenicol for the treatment of animals used for food production.

Using the RIDASCREEN® CAP-Glucuronid assay, it is possible to detect semiquantitatively the metabolized glucuronide form of chloramphenicol, which is mainly contained in urine.

<b>Format:</b>
96 well microtitreplate
 
<b>Detection limit:</b>
urine approx. 300 ng/l (ppt)
 
<b>Time requirement:</b>
incubation time 2 h 30 min (regardless of the number of samples)
 
<b>Sample preparation:</b>
serum: ethyl acetate extraction, centrifugation, evaporation
urine: dilution
 
<b>Recovery rate:</b>
serum (plasma): approx. 72 %
urine: approx. 94 %
 
<b>Cross reaction:</b>
Chloramphenicol 100 %
Chloramphenicol glucuronide (urine) 100 %
in buffer system approx. 200 % CAP glucuronide are detectable
all other cross reactions are < 0.1 %
 
<b>Samples per kit:</b>
dependent on the cut-off standards used up to 46 samples
 
<b>Standards:</b>
0
0.1 ppb and 0.2 ppb for urine
0.5 ppb for urine and serum (plasma)
2.0 ppb for serum (plasma)
 
<b>Measurement: </b>
microtiter plate reader (450 nm)