Vitamin B7 or Vitamin H or Biotin

Biotin, also known as vitamin H, is an important nutritional factor and food additive, playing a major role in the metabolism of both humans and animals alike. Biotin acts as a coenzyme and participates in the process of gluconeogenesis, biosynthesis of fatty acids and the metabolism of amino acids.
Biotin deficiency in mammals leads to growth inhibition, loss of appetite and reduced utilization of food components. Advanced stages of avitaminosis manifest themselves in hair loss, dermatitis and signs of paralysis. The biotin requirement for adults is between 100 and 200 mg per day.

Using the RIDASCREEN® Biotin assay, it is possible to detect biotin in vitamin enriched food, feed, and drugs rapidly and with accuracy.

  
Format: 96 well microtitreplate

Detection limit:
milk: approx. 0.3 µg/kg (ppb)
milk powder: approx. 3 µg/kg (ppb)
grain products: approx. 1.2 µg/kg (ppb)
vitamin powder, -tablets, -juice: approx. 120 ng/kg (ppt) 
 
Time requirement: incubation time 1 h 30 min 
 
Sample preparation: homogenization (optional digestion with alpha-Amylase), precipitation (optional Ascorbase-treatment)
 
Cross reaction:
Biotin 100 %
Biotin-4-Amidobenzoate 89 %
Biocytin 83 %

Samples per kit: up to 42 
 
Standards: 0 / 12 / 37 / 111 / 333 / 1,000 ppt 
 
Measurement: microtiter plate reader (450 nm)